Hét forum over de ziekte van Lyme (Lyme-Borreliose)
https://www.lymeforum.nl/forum/
VerlorengezondheidM schreef:Als ik jou was zou ik ook Babesia Microti mee laten testen.
In ons laboratorium testen we op antilichamen door de SeraSpot, IFT en ELISA-technieken. In het algemeen, heeft de SeraSpot een hogere sensitiviteit en specificiteit bij het detecteren van Borrelia antilichamen in vergelijking met de ELISA en IFT testtechniek.
Sproetje schreef:Ga het bespreken en vraag meteen even naar die IFA van Chroniker Labor
How to test for Lyme Disease?
One of the ways Lyme Disease is diagnosed is by the infamous red “bulls eye” rash. Although this rash does occur in the event of Lyme Disease from a tick bite, it is not always
There are 2 ways to test for Lyme Disease infection: A Serology test and a Direct Test.
Serology: A serology test relies on the performance of your immune system to detect the presence of antibodies (immune response) to an antigen (germ/bacteria/spirochete) like Borrelia Burgdorferi.
Direct: A direct test looks for the presence of the spirochetes themselves.
Serology: You are looking for the evidence/smoking gun in the crime.
Direct: You are looking for the actual criminal.
This becomes extremely important to understand because not all immune systems are created equal! However, if the criminal (spirochete) has been in the house (your body), then a direct test like a direct IFA (immunofluorescence assay), Blood Culture or a FISH (fluorescence in situ hybridization) test should find the culprit (8,9,10,11). Indirect tests like the ELISA has been estimated to miss as many as 35% of all culture proven Lyme Disease infections (12). In any case, the take-away lesson here is: if you think you have Lyme disease talk to your doctor about laboratory studies that perform DIRECT TESTING on your blood.
Lyme Disease is ultimately immunosuppressive, and so the longer it goes on undetected, the less likely you will get a positive result from a standard ELISA or Western Blot (serology) lab test. The International Lyme and Associated Diseases Society reports that “Of patients with acute culture-proven Lyme disease 20-30% remain seronegative on serial western blot sampling”(12). In plain English that means that the sickest patients have Borrelia Burgdorferi spirochetes grown out of their blood even though the ELISA and Western Blot serology tests came back negative for Lyme. In the laboratory world this is called a “false negative test result”.
According to the Centers for Disease Control, Lyme disease is the fastest growing vector borne (from insects) infectious disease in the United States (13,14). The CDC reports that 300,000 cases of Lyme disease are reported annually. As you can imagine, when left undiagnosed, these corkscrew shaped bacteria can wreak havoc in the body becoming the “great imitator” of many diseases for all the misery they cause. They can wiggle their way in to your heart and brain, and the results are devastating to your health and mental wellness. If you think you have Lyme Disease, please see a doctor and ask for a direct antigen test for Borrelia Burgdorferi.
Tune in next month for our best tick bite prevention strategies!
Direct vs indirect immunofluorescence
Understand the difference between direct and indirect methods for immunofluorescence.
Immunofluorescence (IF) or cell imaging techniques rely on the use of antibodies to label a specific target antigen with a fluorescent dye (also called fluorophores or fluorochromes) such as fluorescein isothiocyanate (FITC). Antibodies that are chemically conjugated to fluorophores are commonly used in IF.
.....
Sproetje schreef:Dan is direct IFA een combinatie van antistoffen en fluorescent microscopische techniek?