http://www.sciencedirect.com/science/ar ... 9X15300327
Occurrence of Borrelia burgdorferi s.l. in different genera of mosquitoes (Culicidae) in Central Europe
Door: Christian Melauna, Sina Zotzmanna, Vanesa Garcia Santaellaa, Antje Werblowa, Helga Zumkowski-Xylanderb, Peter Kraiczyc, Sven Klimpela,
Gepubliceerd: 12 Nov. 2015
Abstract
Lyme disease or Lyme borreliosis is a vector-borne infectious disease caused by spirochetes of the Borrelia burgdorferi sensu lato complex. Some stages of the borrelial transmission cycle in ticks (transstadial, feeding and co-feeding) can potentially occur also in insects, particularly in mosquitoes. In the present study, adult as well as larvae mosquitoes were collected at 42 different geographical locations throughout Germany. This is the first study, in which German mosquitoes were analysed for the presence of Borrelia spp. Targeting two specific borrelial genes, flaB and ospA encoding for the subunit B of flagellin and the outer surface protein A, the results show that DNA of B. afzelii, B. bavariensis and B. garinii could be detected in ten Culicidae species comprising four distinct genera (Aedes, Culiseta, Culex, and Ochlerotatus). Positive samples also include adult specimens raised in the laboratory from wild-caught larvae indicating that transstadial and/or transovarial transmission might occur within a given mosquito population.
